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Aging-associated decline in phagocytosis may be due to the production of reactive oxygen species (ROS) from neutrophils, which is well known to destroy ingested material. In the present study, we investigated whether ROS scavengers have a beneficial effect on the phagocytosis of chlorhexidine gel by neutrophils from aged mice. Chlorhexidine gel was well phagocytosed by neutrophils isolated from young mice. However, neutrophils from aged mice had an impaired phagocytosis of chlorhexidine gel. Ascorbic acid (5 mM) and N-acetylcysteine (1 mM) restored the phagocytosis by neutrophils from aged mice. These results indicate that ROS scavengers restore the phagocytosis of neutrophils from aged mice.The goal of this project is to understand the pathophysiology of the afferent component of diabetic neuropathy in order to develop new treatments. In the previous funding period we developed the "spared nerve injury" (SNI) model of diabetes neuropathy in rats. This model recapitulates many of the clinical and histopathological features of diabetic neuropathy, including the painful symptoms, distal hypoalgesia, and histopathology of diabetic polyneuropathy. We also showed that peripheral nerve growth factor (NGF) is necessary for the development of hyperalgesia, that there is a critical balance between NGF and glial cell line-derived neurotrophic factor (GDNF) in the development of neuropathic pain, and that nerve crush causes a spinal protein synthesis/synthesis/secretion-dependent increase in NGF that contributes to the development of neuropathic pain. We are also investigating the role of retinal photoreceptors in the development of neuropathic pain and diabetic retinopathy. In the current funding period we propose to 1) determine whether NGF is the key mediator of diabetic neuropathic pain; 2) determine whether GDNF plays a role in the development of diabetic neuropathic pain; 3) determine whether changes in gene expression in the spinal cord (c-fos, PGE2, COX-2, and EP3) are correlated with 1a423ce670 Video To 3GP Converter 2.0.2.10 With License Key [April-2022] 2-3.5/5 Windows/Mac OS X (32/64-bit) Video to 3GP Converter tool supports the following input files: AVI, DIVX, MPG, MP4, MOV, RM, RMVB, RMVD, VOB, WMV. Video to 3GP Converter has some of the features you might want to take advantage of when you are going to convert video to 3GP format, such as batch conversion, transition, background music, album preview. In the video player, you can preview the video and then trim the video clips you want to keep. Features: Import clips from most of the popular video formats, such as AVI, MPG, MP4, VOB, RMVB, VIVO, WMV. 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